Mixtures of Matrigel with CM from H460 KrasQ61H lung cancer cells manipulated for expression of YY1, ZNF322A and/or Shh were injected subcutaneously into nude mice and then the Matrigel plugs were collected on day 10 for macroscopic analysis and IHC staining of CD31 to reveal blood vessel infiltration. Here, SHH is linked to lung carcinoma.