PECAM1 and lung carcinoma: Mixtures of Matrigel with CM from H460 KrasQ61H lung cancer cells manipulated for expression of YY1, ZNF322A and/or Shh were injected subcutaneously into nude mice and then the Matrigel plugs were collected on day 10 for macroscopic analysis and IHC staining of CD31 to reveal blood vessel infiltration.