Compared to the AKI group, eNOS was upregulated in all three treatment groups: EVs alone (0.35 ± 0.11 vs. 0.97 ± 0.02 normalized expression, p < 0.05), pFUS alone (0.35 ± 0.11 vs. 1.12 ± 0.14 normalized expression, p < 0.05), and pFUS + EVs (0.35 ± 0.11 vs. 0.85 ± 0.46 normalized expression, p > 0.05) (Fig. 6c). This evidence concerns the gene NOS3 and acute kidney injury.