Compared to the AKI group, SIRT3 was modestly upregulated by EVs alone (0.76 ± 0.37 vs. 1.18 ± 0.19 normalized expression, p > 0.05) and significantly upregulated by pFUS alone (0.76 ± 0.37 vs. 2.19 ± 0.27 normalized expression, p < 0.05). Here, SIRT3 is linked to acute kidney injury.