On the other hand, compared to the AKI group, pro-apoptotic genes like BAX and Caspase-3 were downregulated by both EVs alone (BAX 0.10 ± 0.01 vs. 0.15 ± 0.06 normalized expression, p < 0.05; Caspase-3 1.63 ± 0.24 vs. 2.99 ± 0.95 normalized expression, p > 0.05) and by pFUS alone (BAX 0.07 ± 0.01 vs. 0.15 ± 0.06 normalized expression, p > 0.05; Caspase-3 1.29 ± 0.53 vs. 2.99 ± 0.94 normalized expression, p > 0.05). The gene discussed is CASP3; the disease is acute kidney injury.