Trachelogenin (5–10 μM), a lignan isolated from Combretum fruticosum, promoted autophagic cell death, but not apoptosis, as shown by the appearance of AVOs and autophagic vacuoles, by the increase in Beclin 1 expression and by the conversion of LC3 I into LC3 II in HCT-116 colon cancer cells [266]. The gene discussed is BECN1; the disease is malignant colon neoplasm.