Subsequent cultivation within Dulbecco’s Modified Eagle’s Medium/Ham’s -F12 supplemented with epidermal growth factor (EGF), fibroblast growth factor 2 (FGF-2), B27 and 10% fetal calf serum (FCS) led to an adherently growing cell culture for both tumor tissue samples (Figure 3D,E). This evidence concerns the gene EGF and neoplasm.