FUT9 and colonic neoplasm: To confirm the direct involvement of FUT9 in the CSC-like phenotype of human colon cancer cell lines, we proceeded with CRISPR-Cas9-mediated knock-out (KO) of FUT9 in the top two FUT9-High cell lines (Figure 4A), KM12 (CMS1 subtype) and SW1116 (CMS2 subtype) cells.