To confirm the direct involvement of FUT9 in the CSC-like phenotype of human colon cancer cell lines, we proceeded with CRISPR-Cas9-mediated knock-out (KO) of FUT9 in the top two FUT9-High cell lines (Figure 4A), KM12 (CMS1 subtype) and SW1116 (CMS2 subtype) cells. The gene discussed is FUT9; the disease is malignant colon neoplasm.