With the immunophenotyping of CSLC, the anti-CD133 antibody was found to be effective for isolating a population of colon cancer cells that retained the properties of stem cells (CSLC), while anti-cytokeratin 20 (CK20) and anti-Mucin-2 (MUC2) were specific epithelium colonic differenziation markers. The gene discussed is KRT20; the disease is malignant colon neoplasm.