To determine whether depletion of endogenous PDPK1 has any effect on the proliferation and survival of PCa cells that exhibit active PDPK1, we performed lentiviral shRNAs‐mediated knock‐down of PDPK1 in a panel of PCa and non‐transformed prostate epithelial cells. Here, PDPK1 is linked to posterior cortical atrophy.