Significantly, the indicators related to EMT process (N‐cadherin, vimentin, E‐cadherin) were consequentially affected when AKR1B10P1 depressed, and the immunofluorescence assay definitely proved a suppressive effect on EMT in HCC cells through AKR1B10P1 inhibition. Here, AKR1B10P1 is linked to hepatocellular carcinoma.