AKR1B10P1 and hepatocellular carcinoma: Mouse xenograft model was constructed orthotopically to verify the effect of AKR1B10P1 on HCC tumorigenesis in vivo. Quantification of the xenografted liver weight 6 weeks later after the injection illustrated that effective AKR1B10P1 depletion in Hep3B cells led to less tumour formation in mice (Figure 4A and B, Figure S3A).