A classifier established by the combination of miR-15b and miR-130b had an AUROC of 0.980 (96.7% sensitivity and 91.5% specificity) for detecting ANHC (44), and this miRNA classifier could identify 44 of 45 (97.8%) HCC cases with tumor-node-metastasis stages I and II, whereas serum AFP (cutoff value 20 ng/ml) could only detect 22 of 45 (48.9%) of the same cases; also, miR-15b and miR-130b were markedly reduced after surgery. Here, AFP is linked to hepatocellular carcinoma.