An integrated parameter AFP/GP73 (Golgi protein 73) was created to magnify the differential diagnosis of ANHC in terms of better sensitivity and specificity (66), which had an AUROC of 0.662, sensitivity of 68.6%, and specificity of 58.8% for differentiating ANHC from LC and an AUROC of 0.796, sensitivity of 81.4%, and specificity of 70.0% for differentiating metastatic ANHC from adenocarcinomas; these values were slightly higher than those of GP73 alone (AUROC of 0.747, sensitivity of 57.7%, and specificity of 87.0%). This evidence concerns the gene AFP and laryngotracheoesophageal cleft.