The combination of serum SPARC and THBS2 for distinguishing HCC (n = 44) with an AUROC of 0.97, sensitivity of 86%, and specificity of 100% and ANHC (n = 22) with an AUROC of 0.95, sensitivity of 91%, and specificity of 93% from healthy controls (n = 30) (170), and HCC patients with high THBS2 levels had significantly shorter disease-free survival and overall survival than those with low THBS2 levels, indicating that serum THBS2 could be used as a novel indicator for a poor prognosis of HCC. This evidence concerns the gene SPARC and hepatocellular carcinoma.