Although ST2 deficiency did not affect the infiltration of CD8+ T cells and myeloid cells into the tumors, it did increase the frequency of tumor-infiltrating Tregs.26 At last, one study shows inhibiting effects of IL-33 on colon cancer cell growth,45 however, we did not observe direct effects of IL-33 on CT26 cell proliferation (Supplementary Fig. 3). Here, CD8A is linked to malignant colon neoplasm.