Next, we used reliable real-time cell proliferation monitoring technology to monitor the proliferation of the EGFR-wild type lung cancer cell line H838 (both ELISA and Western blot results indicated positive EPO-R expression) and the EGFR mutation cell line H1650 (only positive via Western blot) in the presence of rHuEPO or physiological buffer control (PBS). The gene discussed is EGFR; the disease is lung carcinoma.