Serum insulin levels (CON: 2385 ± 193.8 pg/ml vs. T2DM-SED: 3250 ± 159.8 pg/ml, p < 0.01) (Figure 3D) and TG (CON: 2.82 ± 0.04 mmol/l vs. T2DM-SED: 3.76 ± 0.13 mmol/l, p < 0.01) (Table 1) were increased in T2DM-SED mice compared with those of CON mice but were decreased in the T2DM-HIIT group (T2DM-SED vs. T2DM-HIIT: 2258 ± 229.7 pg/ml; T2DM-SED vs. T2DM-HIIT: 3.21 ± 0.06 mmol/l, p < 0.01). This evidence concerns the gene INS and type 2 diabetes mellitus.