Therefore, we observe the effect of platelet lysates on those signaling, and found that platelet lysates from patients after RFA could promote epithelial-mesenchymal transition and Akt, ERK1/2 and Smad3 signaling activation, which suggested platelet lysates may facilitate tumor metastasis and vasculogenic mimicry through Akt/ERK1/2/Smad3 signaling mediating epithelial-mesenchymal transition. This evidence concerns the gene SMAD3 and neoplasm.