From that time, AKT-mediated phosphorylation of TWIST1 is already shown (Alexander et al., 2006), we wanted to investigate the effect of our mutants on growth, metastasis, and induction of TWIST1 induced Vimentin and N-cadherin in breast cancer cells 4T1 and 67NR cells as well. This evidence concerns the gene CDH2 and breast carcinoma.