Since we hypothesized that Akt-mediated phosphorylations of TWIST1 would affect EMT in mouse breast cancer cell lines, and alanine mutants of TWIST1 interfered with metastasis of 4T1 cells we wanted to know whether ectopic expression of alanine mutants affected the expressions of N-cadherin and vimentin. The gene discussed is VIM; the disease is breast carcinoma.