To investigate the role of Tregs in the maintenance of the convalescence state during the late phase of cGN, we depleted Tregs using “depletion of regulatory T cell” DEREG mice expressing the diphtheria toxin (DT) receptor under the control of the FoxP3 gene promoter.24 Treg depletion at the early phase ameliorated cGN.15,23 To verify the roles of Tregs at the late or convalescence stage, we assessed the depletion of Tregs 21 days after nephrotoxic antibody injection (Fig. 1c–e). This evidence concerns the gene FOXP3 and childhood gender nonconformity.