Recent single-cell transcriptomics has revealed the trajectories of tissue adaptation in mice and humans; for example, the priming of Tregs to nonlymphoid tissues occurs in the LNs.11,13 Previous studies demonstrated the importance of CCR6 and CXCR3 in Tregs for the attenuation of cGN symptoms at the fulminant phase.16,20,21 In addition to the chemokine receptors demonstrated by these studies, we proposed that CCR4 signals in Tregs are important for the migration of Tregs from renal LNs into the kidney, especially during the late stage. The gene discussed is CCR4; the disease is childhood gender nonconformity.