RORC and kidney disorder: GATA3 expression is detected in most tissue Tregs,11 T-bet (Tbx21) is detected in a subset of muscle and colonic Tregs25,26 and RORγt (Rorc) is detected in distinct subpopulations of colonic and other tissue Tregs.27–29 Thus, we measured GATA3+, RORγt+, and T-bet+ factions in Tregs during the course of kidney disease development (Fig. 2d).