These genes included AHNAK2, TP53, PLEC, COL5A1, FAT3, PCDH17, RIMS1, ASH1L, CDH23, DNAH17, DYNC2H1, MYCBP2, SLIT3, TRRAP, and USH2A. Interestingly, we found 9 of the 15 genes whose mutations were significantly associated with worse OS in pan-cancer (log-rank test, p < 0.05) (Supplementary Fig. 3). This evidence concerns the gene PLEC and cancer.