And from the results of western blot, high-metastatic HCC cells (97hm and Huhm) have higher expression of mesenchymal associated proteins (N-cadherin, Vimentin, beta-catenin, and Snail) than their parental low-metastatic HCC cells (97h and Huh7) (Fig. 1g). This evidence concerns the gene VIM and hepatocellular carcinoma.