To selectively track and eliminate individual BSK-NUPs, we used CRISPR/Cas9 gene editing to biallelically introduce sequences encoding an auxin-inducible degron (AID) and a NeonGreen (NG) fluorescent protein to the NUP50, NUP153, and TPR endogenous genomic loci in colorectal adenocarcinoma DLD-1 cells (Fig. 1a and Supplementary Figs. 1a, b and 2). Here, NUP50 is linked to colorectal adenocarcinoma.