In order to test whether modulation of mitochondrial morphology by ammonia represents an early event in ammonia-induced effects in astrocytes, we transfected the human astrocytoma cell line MOG-G-CCM with pEGFP-Mito, a GFP variant targeting the mitochondrial matrix (Weber et al., 2013). The gene discussed is MOG; the disease is astrocytoma (excluding glioblastoma).