We employed HeLa-SIRT4-eGFP cells, a stable cell line overexpressing SIRT4, and the corresponding control cells (HeLa-eGFP) to test whether inhibition of mitochondrial GDH by SIRT4 can also restore mitochondrial respiration and/or mitochondrial morphology upon hyperammonemia. The gene discussed is SIRT4; the disease is Hyperammonemia.