Indeed, overexpression of GDH2 from a plasmid restored the sensitivity of human astrocytoma cells depleted for endogenous GDH2 to ammonia, excluding off-target effects of the GLUD2 siRNA, and further supported that GDH2 is specifically required for ammonia to impair mitochondrial respiration (Fig. 6D). The gene discussed is GLUD2; the disease is astrocytoma (excluding glioblastoma).