TLR4 and P-NF-κB protein levels were significantly increased in the MCAO-Con group (##p < 0.01, #p < 0.05 vs. Sham-Con group), and CSO significantly inhibited the expression of TLR4 and P-NF-κB compared with the MCAO-Con group (*p < 0.05). Here, NFKB1 is linked to craniosynostosis.