The use of multifactorial discriminant analysis based on HbA1c, HDL, LDL, TG, ACR, eGFR, glucose, insulin, QUICKI, HOMA-IR,% S,% B, and eGDR allowed to put forward specific panels of biochemical indicators differentiating patients from T2DM before and after metformin therapy. The gene discussed is INS; the disease is type 2 diabetes mellitus.