Based on the initial statistical analysis, a set of 10 biochemical variables that greatly differ in healthy subjects and T2DM patients: HOMA-IR, QUICKl, ACR, eGFR, HbA1c, eGDR, HOMA-B, HOMA-S, insulin concentration, and TG/HDL ratio, which, together with the plasma levels of adropin, irisin, and vaspin, was selected to further tests using three-parameter discriminant analysis. This evidence concerns the gene FNDC5 and type 2 diabetes mellitus.