Our experimental dataset included proteins that were determined as LC and CRC markers: TSPAN1, LGALS3BP, SLC1A5, and GPRC5A were determined in HT29 and HCT116 line-derived EVs [23], YWHAZ was revealed in HTC116-derived vesicles [32], and ICAM1, PTGFRN, DMBT1, and FN1 were identified in NCI-H23-derived EVs [33], as well as the diagnostically valuable KRAS and EGFR. The gene discussed is TSPAN1; the disease is colorectal carcinoma.