We further demonstrated that the expression of H2AX was significantly higher in tumors treated with the thermo-chemotherapeutic treatment than in those treated with either therapies alone or those not treated at all (untreated control tumors), both at early and late post-observation times (day 9 and day 28 after the first tumor therapy, p < 0.001 and p < 0.01, respectively). This evidence concerns the gene H2AX and neoplasm.