Immunofluorescence microscopy clearly confirmed the drastic reduction of ApoE in the mdx-4cv spleen (Figures 6E, 6F, and 6J) and indicated that the expression of the short spleen-associated isoform of dystrophin is not affected in dystrophinopathy (Figures 6C, 6D, and 6I). This evidence concerns the gene APOE and neuromuscular disease caused by qualitative or quantitative defects of dystrophin.