DMD and neuromuscular disease caused by qualitative or quantitative defects of dystrophin: Immunofluorescence microscopy clearly confirmed the drastic reduction of ApoE in the mdx-4cv spleen (Figures 6E, 6F, and 6J) and indicated that the expression of the short spleen-associated isoform of dystrophin is not affected in dystrophinopathy (Figures 6C, 6D, and 6I).