In agreement with established splenic changes in dystrophic animal models of Duchenne muscular dystrophy, such as morphological adaptations in the white pulp domain of the spleen (Santos et al., 2013) and altered levels of immune cells in the spleen and enhanced migration of inflammatory cells from the splenic reservoir to dystrophic muscle tissues (Farini et al., 2016; Giordano et al., 2015; Mojumdar et al., 2014, 2016; Ouisse et al., 2019), this investigation has established considerable effects on the spleen proteome owing to deficiency in the full-length dystrophin isoform Dp427-M. Here, DMD is linked to Duchenne muscular dystrophy.