First, followed by multivariate cox regression, the optimal 10 prognostic signatures in diffuse type GC samples (Table 1), including: “RALA”, “DDX3Y”, “SRSF5”, “SLC9A3R1”, “GMDS”, “LEF1”, “RFX5”, “CAST”, “FRZB” and “SELP”, were picked out. The gene discussed is RFX5; the disease is gastric cancer.