The highest allele frequency mutations matching tumor content were detected in PTEN (patient EOC49) and NF1 (patient EOC429), which cause overactivation of the mTOR pathway.20,22 Patients with the activated mTOR pathway have been shown to be responsive to phosphatidylinositol 3-kinase/mTOR inhibitors.22 We validated overactivity of the mTOR pathway in the two patients with IHC from tumor tissue samples (Fig 3; Data Supplement). The gene discussed is PTEN; the disease is neoplasm.