By overexpressing MTSS1 in highly malignant human glioma cells, we discovered a role for MTSS1 in suppressing the proliferation and invasion of glioma cells, and we showed that MTSS1 participated in transforming growth factor-beta 1 (TGF‐β1) -induced epithelial‐mesenchymal transition (EMT) in glioma cells. The gene discussed is MTSS1; the disease is central nervous system cancer.