EGFR and non-small cell lung carcinoma: With immunofluorescent staining, the accumulation of green fluorescent protein-LC3 punta was observed in PC9/E cell compared with PC9 cell, as shown in Figure 2D. Collectively, the results showed that mTORC1 was activated in the in EGFR TKI-sensitive and resistant NSCLC Cell lines and autophagy was induced in EGFR-TKI resistance PC9/E.