CPT1A and cancer: To address the question if upregulation of CPT1A is necessary for cancer cells to utilize the exogenous fatty acids, control and CPT1A knockdown PT130 and SW480 cells were treated with OA for 24 h (the loading phase) and subsequently cultured in low glucose media for additional 24–48 h (the unloading phase) to allow the preferential utilization of fatty acids.