We combined linkage analysis, using P. maniculatus and P. polionotus, the polymorphisms between the two species, and a candidate gene approach to link the Dominant spot trait with Sox10. Mutations in Sox10 are known to cause belly spotting and megacolon in Mus and Waardenburg syndrome, types 2E and 4C in humans [52–54]. This evidence concerns the gene SOX10 and Waardenburg syndrome.