In addition, in another public transcript data set (GSE76342) for the colon cancer cell line LoVo with or without metformin treatment, we found that metformin inhibited expression of the CSC markers Lgr5, ASCL2, EPHB3, OLFM4, BMI1, Lrig1, TERT, CD44, and CD133 (Figure 1B). The gene discussed is LRIG1; the disease is colonic neoplasm.