To demonstrate increased activity of the MVA pathway in CSCs of CRC, we cultured tumor spheres and found significantly elevated protein and mRNA levels of key enzymes of the MVA pathway, including HMGCR, FDPS, GGPS1, and SQLE, in tumor spheroids compared to 2D adherent cultured cells (Figure 2B,C). The gene discussed is HMGCR; the disease is colorectal carcinoma.