Kazanjian et al revealed a DHPS gene mutation rate of 6.7% (1/15) in AIDS-PCP in Beijing[30], Deng et al identified DHPS mutations in 12.0% (3/25) patients from Guangzhou[31], the prevalence of P. jirovecii DHPS mutations in this study is not significantly higher than those of these previous studies (P = 0.858, P = 0.137, respectively). Here, DHPS is linked to AIDS.