(A) A schematic diagram illustrating a microfluidics-based GBM-on-a-Chip model to investigate 1 the interactions of immune cell (CD8+ T-cells) with brain microvessels, 2 tumor-associated macrophages (TAMs) and 3 GBM tumor cells in an engineered 3D brain-mimicking ECM. This evidence concerns the gene CD8A and glioblastoma.