The ligands, including WNT7A, WNT7B, WNT3A, WNT5A, and WNT2; the receptors, including FZD2, FZD6, and FZD10; and the transcription factor, LEF1, in the WNT signaling pathway, were significantly upregulated in ESCC (Figure 3(b)). Here, WNT5A is linked to esophageal squamous cell carcinoma.