TNF and infection: Figure 2 shows the production of TNF-α, IFN-γ, IL-12, IL-4, and IL-10 concentrations by RAW 264.7 macrophages in 24 and 48 hours after infection with Leishmania amazonensis and the subsequent treatment with different concentrations of the extract (0.1, 0.5, 1, 5, 10, and 15 mg/mL).