In this manuscript, we illustrated that inflammatory effects of CIA cartilage on CIA FLS in this coculture system is involved with the CD147-mediated NF-κB pathway and showed a feasible inflammatory pathway between cartilage and FLS : CypB/CD147-NF-κB. This newly designed coculture system might guide the optimized study of cartilage-FLS communication in RA. The gene discussed is PPIB; the disease is rheumatoid arthritis.