To address the limited tissue coverage by TMAs, we scored and averaged collagen area, CD8 and TIM-3 marker counts from five areas of whole sections of lung tumor tissues encompassing the inner and peripheral tumor regions, and observed a stronger inverse correlation between collagen I (r = −0.693, p = 0.0308) and collagen III (r = −0.7416, P = 0.0178) area versus CD8 T cell marker count (Fig. 7c). Here, CD8A is linked to neoplasm.