Since BRD9 showed a relatively lower expression in Hep3B cells and a higher level in HCCLM3 cells among the five HCC cell lines (Fig. 1d, e), Hep3B cells with a stable BRD9 overexpression (Hep3B-BRD9, Supplementary Fig. 4A, B) and HCCLM3 cells with a stable BRD9 knockdown (HCCLM3-BRD9 shRNA, Supplementary Fig. 4C, D) were established. The gene discussed is BRD9; the disease is hepatocellular carcinoma.