To further confirm the role of NEK2 in promoting oncogenesis in cervical cancer, we constructed stable NEK2-knockdown SiHa cells using NEK2 shRNAs (Fig. 3a) and found that shRNAs-mediated knockdown of NEK2 significantly inhibits cervical cancer cell growth and proliferation (Fig. 3b and c), which is consistent with above findings. This evidence concerns the gene NEK2 and cervical cancer.