In the validation with qPCR, we identified that, compared to HF, the HF-EPA group had significantly lower expression of genes positively correlated with adipose tissue hypertrophy and inflammation, such as Arachidonate 5-lipoxygenase (Alox5), Myeloid differentiation protein 88 (Myd88), Stat1, Ccr5, and Card11 (p ≤ 0.05, Figure 2A). The gene discussed is ALOX5; the disease is hydrops fetalis.