FANCD2 and esophageal squamous cell carcinoma: Consistently, FANCD2 protein expression was upregulated (>3-fold) in three ESCC cell lines (KYSE30TSI, KYSE150 and KYSE450) compared with the non-tumorigenic immortalized esophageal epithelial cell line NE1, as shown by Western blotting (Figure 1b and Figure S1).