RASAL1 and breast cancer: Rare/novel heterozygous damaging variants were also identified in RASAL1 including: (i) the premature stop codon c.158G > A p.Trp53Ter (found in cis with the novel missense change p.Ile83Asn); (ii) a novel frameshift p.Leu292CysfsTer5 in another patient; (iii) the novel missense changes, predicted damaging, p.Ala43Thr and p.Ser466Asn in two unrelated patients (Table 3), all pointing to a loss-of-function mechanism for RASAL1 in BC, in concordance with previous data indicating that it might act as a tumor suppressor gene in thyroid cancer [24].