Accordingly, this further confirmed that the therapeutic effect of 2 Hz EA on SNI-induced neuropathic pain is highly correlated with the BDNF/TrκB cascade, implying that 2 Hz EA stimulation induces the analgesic effect via blocking the BDNF/TrκB signaling pathway in the spinal cord of SNI rats. The gene discussed is BDNF; the disease is neuropathic pain.