To test whether any of another viral protein(s) of MMDaV is responsible for the differential localization of V2 associated with MMDaV infection, agrobacteria cultures harboring the construct of GFP-V2 was transiently infiltrated into RFP-H2B plant leaves together with bacterium cultures containing pCHF3 plasmids expressing each of the viral proteins including V1, V3, V4, V5, RepA, and Rep independently. This evidence concerns the gene H2BC21 and infection.