Result revealed that a higher percentage of F4/80+/Arg1+ macrophages and a lower percentage of F4/80+/iNOS+ macrophages in biofilm-infected tissues (7 days post-infection) than those in planktonic bacteria-infected tissues (1 day post-infection; Figures 1D,E), reflecting a less M1-like phenotype of macrophages under a biofilm-associated infection. Here, NOS2 is linked to infection.