The novel findings of this study included that: (1) insulin-deficient diabetic mice with mild hyperglycemia had an increased vulnerability to AF induction; (2) restoration of normo-glycemia in T1Dx animals by in vivo insulin treatment reduced AF vulnerability and propensity; (3) insulin-deficient diabetic mice displayed impairment in the expression and translocation of the major insulin-sensitive GLUT isoforms in the atria, which was rescued by in vivo insulin treatment. Here, INS is linked to atrial fibrillation.