SHMT2 and non-small cell lung carcinoma: Western blotting for SHMT2 in a set of established cell lines (LPC43, HeLa, A549, HEK392, and MCF-7) showed that NSCLC-derived LPC43 has relatively high endogenous SHMT2 expression, whereas SHMT2 protein is relatively low in HeLa (Fig 1B).