For this purpose, we selectively knocked-down the expression of pink1 and pdr-1 using RNAi in the models of premature aging (wrn-1 (gk99)), Supplementary Figures 2, 3 and neurodegenerative disease (NL5901 and UM0001), and evaluated the effect of spermidine. This evidence concerns the gene PINK1 and neurodegenerative disease.