Similar to data obtained from RNA-sequencing, qRT-PCR validated that ZIKV infection induces up-regulation of interferon gamma induced protein 10 (IP-10), interleukin-6 (IL-6), 2'-5'-oligoadenylate synthetase 1 (OAS1) and interferon-β (IFN-β) genes (Figure 2(D)). This evidence concerns the gene IFNB1 and Zika virus infectious disease.