Besides, Western blotting revealed that miR‐5188 inhibition led to reduced protein levels of P‐PI3K, P‐AKT, CCND1, CDK4 and c‐JUN, but increased protein levels of FOXO1 in glioma cells with SP1 overexpression (Figure 6D), suggesting that miR‐5188 was enhanced by SP1 through the PI3K/AKT pathway. This evidence concerns the gene FOXO1 and central nervous system cancer.